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Biochemistry Review

Horizontales
Type of inhibition
Solution that can resist pH change upon the addition of an acidic or basic components
First step of PCR; uses high heat
Mathematical term to describe the rate of DNA production in PCR
Sequence EcoR1 recognizes
Chemical that allows you to separate proteins on size, removing the effect of charge
Lower the activation energy of a chemical reaction and increase the rate of a chemical reaction without being consumed in the process
Comassie stain color for post-PAGE stain
Short nucleic acid sequence that provides a starting point for DNA synthesis
Method to organize and write introduction of paper
Machine that is able to rapidly change temperatures for set amount of times.
Material commonly used for protein gels
Second step of PCR
Binds to guanine
PH where molecule is neutral
Attracted to positives
Verticales
Movement of charged particles in an electric field
Type of inhibition
Most important thing to remember when writing
Uses electric shock to allow plasmid into bacteria
Equation used to determine how much light will be absorbed by a solution
Small autonomously replicating pieces of circular DNA
Blot used to identify proteins
Common coating on TLC plates
Mathematical equation used to determine unknown concentrations
Constant; 6.626 x10^-34 J*s
Enzyme that cuts DNA at specific sequences
First step in enzyme purification
Useful to create multiple copies of DNA rapidly
Better gel material for DNA
Not sticky ends for a restriction enzyme
Used in many applications to bind and ultimately target specific proteins
Third step of PCR; step where you actually make DNA
Gene that produces beta- galactosidase and makes bacteria grow blue on plates with X-gal
C21H20BrN3