Molecular; main use of SDS-PAGE is for this; not quite the spelling of your professor's last name
Blue; Loading dye
Word to describe the rate of particle movement with increasing concentrations of polyacrylamide. Also how children walk when they know they've misbehaved.
Binds to the proteins in a constant ratio of 1.4 g/ gram of protein, covering proteins with negative charges
Long polymers of acrylamide
First step in making a gel
The movement of charged particles in an electric field
Section of the gel that is used to compress all the proteins into a thin band.
Q; can change if pH of buffer is altered
E; Increase this, and your molecules will be separated faster, albeit with an increased chance of "streaking"
Blue; Used after an electrophoresis is over and stains all proteins
Need to reach the ___ point during the gel preparation
Such a simple tool, yet so important when casting the gel. Hope there aren't any leaks, but you'll find out soon enough if this isn't properly used!
Left-handed; part of the agarose chain that intertwine with each other
Verticales
If you use this to make your gel, the sample will not separate. However, this is required for life.
Being immersed in this reduces heat during separation
Word to describe the agarose gels because nothing in system denatures a protein
Beta version; smells like dead fish, so use it under a hood. Reduces the number of disulfide bridges
Part of the amino acid that gives each protein a unique charge character, as well as size and shape.
Electrophoresis separates proteins based on size, charge and this
Word to describe physical material of agarose gels
Usually loaded in the first lane; used to approximate size of bands
Ratio of velocity of particle divided by the applied voltage
Caused by ammonium persulfate, and used to quickly link the acrylamide molecules together
Coefficient used to describe the retarding effect of size and shape of the particle
Shape that moves faster than rods during separation; quantify surface area as 4*pi*r^2
Agarose is a natural polysaccharide of __ and 3,6-anhydrogalactose derived from agar.
Controls the speed of polyacrylamide gels hardening
Also known as resolving or separating gel section; also known as a form of exercise
Most common support for electrophoresis
Used to physically make the lanes in a gel; AKA Ariel's dinglehopper
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There is a 'Make Printable' button on the top left of your puzzle that will let you sign up for a plan or purchase a single puzzle.
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Once you publish your puzzle, you can click the print icon or use your browser’s print function.
It looks weird when printed.
First make sure you’ve published your puzzle. See the 'How do I print?' section above for more information.
99% of other printing issues have to do with printer settings. Instead of trying to fiddle with printer settings, which can be time-consuming and frustrating, there are a couple workarounds you can try.
You can try printing from a different browser, since different browsers have different default print settings. Alternately, you can try saving as a PDF (next to the print icon) and print that.
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We invested in building a number of premium features that free sites are unable to offer: the ability to automatically or manually add words to your puzzle, save-as-you-go puzzle editing, the ability to access your puzzles from any computer, an uncluttered and ad-free interface, the ability for friends and colleagues to solve your puzzles online, and quick responsiveness to reported issues.
My Crossword Maker is the lowest-price service we've found that provides these features, and is designed for people who are not satisfied with what free sites are able to provide.
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We’re constantly trying to provide a clear and straightforward user experience, and we’re not trying to hide the price: on the page where you make your puzzle, the price is listed in the overview in the top left (before you log in), below the 'Make Printable' button (after you log in), in the FAQ below the grid, and also in the tour.
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Not all my words are in my puzzle.
When you hit the 'Arrange' button, our algorithm will try to arrange all your words on the grid, and will move words around to try to fit the most words.
This will get all the words on the grid about 90% of the time, depending on the word list. For the other 10%, you will get an alert explaining that the words that did not fit were added to the scratchpad.
It prints on two pages.
Big puzzles won’t always print on one page — the clues and grid are the smallest we could make them while keeping them readable for everyone.
That said, different browsers print in slightly different ways, so you could try using 'print preview' using a different browser (or try the PDF button) to see if you like how that looks better.
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