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Flow Cytometry Compensation

Horizontales
A mathematical correction for fluorescent spillover
One of the three laser colors on a BD FACSCanto
Range of light emitted in the same wavelength by different fluorescent dyes
An optical device that selectively transmits light of specific wavelengths; examples are band pass, long pass, and short pass
Commonly used name for fluorescein isothiocyanate
A compensation error in which too little compensation is applied; it can result in a false positive population.
One of the three subsystems of a flow cytometer
One of the three laser colors on a BD FACSCanto
Light absorbed by a fluorescent dye; what occurs when laser light interacts with a fluorochrome
A point below which events are not collected; can be used to exclude debris
Longer wavelength photons released by an excited fluorescent dye as it returns to its resting state
Verticales
One of the three laser colors on a BD FACSCanto
A compensation error in which too much compensation is applied; it can appear as a population below an axis
One of the three subsystems of a flow cytometer
Flow rate that results in the lowest CV
The portion of fluorochrome’s spectrum that is measured in another detector
A measured value for the middle of a population that is used in compensation calculations
A technology that simultaneously measures multiple characteristics of single particles, serially, in a rapidly flowing stream
Useful surrogate for cells when compensating rare or dim populations
Compensation controls require a _________ and positive population with equivalent background.
One of the three subsystems of a flow cytometer
Single stained samples used to calculate compensation; can be beads or cells
Refers to a dye that is made of a donor and an acceptor molecule
Sample injection tube; where sample is drawn from tube to flow cell