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Flow Cytometry Basics

Horizontales
Location of interrogation point for event in a flowing stream
Flow Cytometry Subsystem that includes excitation and emission components
This data display uses a two dimensional plotting strategy, plotting a dot for every event
Acronym for parameter that can be used to determine relative complexity or granularity of a particle
Optimal pulse measurement
Flow Cytometry samples must be in this form
Acronym for light measured "forward" of the LASER
This type of plot has # of events on the y-axis
This is monochromatic and unidirectional
T cells and B cells are these
Measurement in Flow Cytometry that can be inherent to a particle or due to an attached tag
Done after data acquisition
Verticales
Flow rate that should be used for low CVs
This pulse measurement is used when applying a threshold
Flow Cytometry Subsystem that converts photons into data
Flow Cytometry Subsystem that delivers cells to interrogation point
A population that can be identified by FSC and SSC alone
When a particle goes through a LASER, this electronic result is created. It is represented by a plot of Time vs. Volts.
A filter that stipulates the wavelengths that pass through by using the following format "488/10"
Optical filter that allows long wavelengths to pass
This biological sample cannot be acquired on a flow cytometer without proper preparation
Quadrant where double positive events fall in a two-dimensional plot
Name for a test that measures 3 major lymphocyte populations
A technology that simultaneously measures multiple characteristics of single particles, serially, in a rapidly flowing stream
Force required for fluid stream on a FACSCanto