______ genes are used in both transcriptional and translational fusions
To detect transcript differences (but not quantitatively)
This may be a cause of cross-hybridization of a probe
Acronym for digoxigenin
How to detect GFP, YFP etc
A basic tool for analyzing sequence similarity
A type of sequence that complicates the isolation of targets by hybridization
This is not a nice thing to say to someone but rather a wonderfully powerful genetic tool: functional __________.
Probes that are very sensitive have high specific________
The Gene ________ is a vocabulary used to classify sequences based on 3 different metrics.
The strandedness of the mRNA
Look for these RNAs to assess whether your RNA preparation is good
Sequencing reads are first joined into one of these
________ sequence tags were used to validate bioinformatic predictions
Verticales
A _______ genome is often use to map sequence reads
__________ transcript abundance is evaluated by northern blotting and other methods.
A sequence of DNA introduced into an organism.
To determine the direction a fragment is inserted into a plasmid, it is useful to know a restriction enzyme that cuts the insert _______.
The phase of a PCR which is used for quantitation assessments.
A DNA fragment containing a modified nucleotide that is detectable.
The reporter gene used for dynamic expression changes
_________ -specific probes can be used to detect either sense and antisense transcripts.
These conditions limit the hybridization between a probe and its possible targets
Klenow ______ is used in random priming.
The most common massively parallel sequencing platform
A short sequence to initiate cDNA synthesis of mRNAs
Restriction maps are either for circular or ________ DNAs.
Massively parallel sequencing was initially called ________ sequencing.
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