vector de clonación
que se integra en un cromosoma
hospedador.
Amplificación artificial de una
secuencia de DNA mediante ciclos
repetidos de separación de cadenas y
replicación.
técnica de
separación de moléculas de ácido
nucleico que consiste en pasar una
corriente eléctrica a través de un gel de
agarosa o de poliacrilamida.
uso de técnicas irt
vitro para el aislamiento, la alteración y
la expresión del DNA y para el desarrollo
de organismos modificados
genéticamente.
Verticales
vector de una sola
copia que puede contener insertos
extremadamente largos de DNA y se
utiliza ampliamente para clonar
segmentos de genomas grandes.
enzima que
reconoce una secuencia específica de
DNA y la corta; sinónimo de
endonucleasa de restricción.
mapa en el que se
muestra la ubicación de los sitios de
corte de las enzimas de restricción en un
segmento de DNA.
apareamiento de bases de
cadenas sencillas de DNA o RNA de dos
fuentes diferentes (pero relacionadas)
para dar un híbrido de doble hélice.
vector de clonación
que contiene las secuencias reguladoras
necesarias para permitir la transcripción
y la traducción de los genes clonados.
técnica por la que
un gen con una mutación específica
puede construirse in vitro.
aislamiento e
incorporación de un fragmento de DNA
en un vector donde se puede replicar.
colección de
segmentos de DNA clonado lo
suficientemente grande para contener al
menos una copia de cada gen de un
organismo concreto. Sinónimo de
biblioteca génica.
segmento de DNA
diseñado artificialmente que
normalmente contiene un gen de
resistencia a antibiótico o algún otro
marcador de interés y está flanqueado
por sitios de restricción convenientes.
gen utilizado en los análisis
genéticos porque el producto que
codifica es fácil de detectar.
Deducir la secuencia
de una molécula de DNA o RNA
mediante una serie de reacciones
químicas.
Vector de (_______): molécula de DNA autorreplicante que se
utiliza para contener genes clonados u
otros segmentos de DNA para ingeniería
genética.
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